Journal: JBMR Plus

Article Title: Effects of graded phosphate deficiency and vitamin D intervention on growth, bone metabolism, and mineralization in a rat model of neonatal-onset metabolic bone disease

doi: 10.1093/jbmrpl/ziag007

Figure Lengend Snippet: Comparative histological analysis of the proximal tibial growth plate. (A-G) Representative H&E-stained sections of the tibial growth plate from neonatal rats under dietary interventions. (A) NP group: normal growth plate architecture. (B) 1/2P group: modest, non-significant expansion. (C) 0P group: markedly expanded hypertrophic zone (HZ) and significantly increased total width. (E) 0P/D group: slight decrease in size. (D) 0Ca (calcium-free) group, (F) 0P/D + D 3 group, and (G) 0P/D + calcitriol group: significantly reduced HZ and total growth plate widths. Scale bar, 400 μm (applicable to all panels). (H) Data are presented as box plots showing the median (center line), the 25th and 75th percentiles (bounds of box), and the minimum and maximum values (whiskers). Individual data points ( n = 8 per group) are overlaid. The HZ width was significantly wider in the 0P group and significantly narrower in the 0Ca, 0P/D + D 3 , and 0P/D + calcitriol groups compared to the NP group. ** p < .001 vs NP. Abbreviations: NP, normal phosphate; 1/2P, low phosphate; 0P, phosphate-free; 0Ca, calcium-free; 0P/D, phosphate/vitamin D-free; 0P/D + D 3 , 0P/D supplemented with supraphysiological vitamin D 3 ; 0P/D + calcitriol, 0P/D supplemented with supraphysiological calcitriol.

Article Snippet: Growth plate morphology was assessed by measuring proliferative (PZ) and hypertrophic (HZ) zone widths using OLYMPUS cellSens Dimension imaging software (Olympus Corporation), with HZ% calculated as HZ/(PZ + HZ)., Tibiae from 6- to 8-wk-old rats were scanned using a SkyScan 1176 micro-CT system (Bruker) at 18 μm isotropic resolution.

Techniques: Staining

Journal: Foods

Article Title: Probiotic Potential of Weizmannia coagulans MA42, an Endospore-Forming Probiotic Bacterium Capable of Dietary Fiber Digestion

doi: 10.3390/foods15040710

Figure Lengend Snippet: Disc diffusion assay of cell-free culture supernatants (CFCSs), obtained from W. coagulans ATCC 7050, W. coagulans MA42, W. coagulans S5, and W. coagulans P13 cultured in mMRS broth at 37 °C under static conditions for 24 h, against E. coli ATCC 25922, S. Typhimurium TISTR 292, B. cereus TISTR 747, and S. aureus TISTR 746. Control (M): uninoculated mMRS broth. Un-neutralized CFCS ( A ) and neutralized CFCS (pH 7) ( B ).

Article Snippet: W. coagulans MA42 was the most effective in inhibiting target pathogens, with 17.50 ± 1.50, 16.00 ± 1.00, 14.50 ± 0.50, and 8.50 ± 0.50 mm clear zones against E. coli ATCC 25922, S. Typhimurium TISTR 292, B. cereus TISTR 747, and S. aureus TISTR 746, respectively, showing an inhibition zone better than W. coagulans ATCC 7050, as shown in A.

Techniques: Diffusion-based Assay, Cell Culture, Control